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75 ml Solution II PEG solution for the transformation of competent cells 150 ml (2 × 75 ml) Solution III Salt solution for washing and plating transformed cells 150 ml (2 × 75 ml) Stab Vials: Pichia and E. coli stabs. Store at 4°C. Strain Genotype Phenotype (Pichia only) X-33 wild-type Mut+ GS115 his4 His–, Mut+ KM71H arg4 aox1::ARG4 MutS, Arg+ GS115/Albumin HIS4 MutS GS115/pPICZ/lacZ ...
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